Efficiency of layered double hydroxide nanoparticle-mediated delivery of siRNA is determined by nucleotide sequence

► Layered double hydroxide (LDH) nanoparticle-mediated siRNA delivery. ► Cellular delivery efficiency of siRNAs using LDH depending on nucleotide sequence. ► dsDNA intercalation into LDH interlayer. ► Three interaction modes between dsDNAs and LDHs. ► The dsDNA:LDH mass ratio at the point of zero charge.