Antibody-antigen interaction on polystyrene: An in situ ellipsometric study

► Ellipsometry was used to monitor the binding of antibodies (IgG) to polystyrene. ► Time resolved values of the adsorbed amount and thickness are presented. ► A structure for the adsorbed antibody layers is proposed. ► Mixed human IgG and anti-human IgG layers were assembled. ► The stability of the adsorbed IgG layers was addressed.