Synthesis, characterization and comparative evaluation of phenoxy ring containing long chain gemini imidazolium and pyridinium amphiphiles

► Eight gemini imidazolium and pyridinium amphiphiles have been synthesized. ► Phenoxy ring containing long chain gemini amphiphiles have lower cmc values compared to other cationic gemini surfactants. ► Gemini amphiphiles demonstrate high DNA binding affinity. ► Amphiphiles form stable lipoplex with DNA. ► Display low cytotoxicity on C6 Glioma cell.