Isolation and partial characterization of a biosurfactant mixture produced by Sphingobacterium sp. isolated from soil

► Crude extract of Sphingobacterium sp. 6.2S reduces surface tension to 22 mN/m. ► There are no previous reports on BS production from Sphingobacterium. ► Fraction A reduces surface tension to 33 mN/m and has a cmc of 0.18 g/L. ► Fraction B reduces surface tension (23 mN/m) and shows a triple break in cmc curves. ► Strain 6.2S produces a BS mixture of phospholipids, lipopeptides and a glycolipid.