Structural organization of plasma membrane lipids isolated from cells cultured as a monolayer and in tissue-like conditions

► Structural organization of plasma membrane lipids extracted from 2D and 3D fibroblasts. ► Influence of cell culture conditions on plasma membrane lipid composition. ► Different order in the hydrophobic and interfacial region of the 2D and 3D lipid bilayer. ► Different domain patterns of the membrane bilayer for 2D and 3D lipid extracts.