کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10410328 | 894432 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Electrochemiluminescent detection of acetylcholine using acetylcholinesterase immobilized in a biomimetic Langmuir-Blodgett nanostructure
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کلمات کلیدی
PBSPVA-SbQElectrochemiluminescence detectionDEAEA.U.ECLLangmuir–Blodgett - Langmuir-BlodgettMonoclonal antibody - آنتی بادی مونوکلونالAChE - آهیdiethyl aminoethyl - اتیل اتیل دی اتیلAcetylcholinesterase - استیل کولین استرازElectrochemiluminescence - الکتروکمی لومینسانسProtein orientation - جهت گیری پروتئینphosphate buffered-saline - فسفات بافر-نمکLangmuir–Blodgett films - فیلمهای لنگمیر-بلوچArbitrary Unit - واحد خودآموزcholine oxidase - کولین اکسیداز
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The performances of bioelectronic devices mainly depend on the properties of the bioactive sensing layer and on the quality of its association to the transducer. The achievement of new biospecific organised ultrathin films, directly interfaced with the transducer and inserting biomolecules in a functional and orientated position, may open an original way in the development of new biomimetic optoelectronic devices. This study deals with the possibility to detect acetylcholine using acetylcholinesterase immobilized at the surface of a miniaturised biomimetic nanostructure by means of an electrochemiluminescent device. The highly organised proteo-lipidic nanostructure has been designed using interfacial liposome fusion and Langmuir-Blodgett techniques. By inserting a non-inhibitor monoclonal antibody in a functional position, this nanostructure is able to sequester acetylcholinesterase (AChE) in a suitable orientation and to maintain the enzyme activity for several months. This molecular assembly has been intimately associated with a performant optical screen-printed choline sensor based on luminol electrochemiluminescence. The linear range for acetylcholine extends over more than two decades, with a detection limit of 4Â ÃÂ 10â7Â M.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 107, Issue 1, 27 May 2005, Pages 82-87
Journal: Sensors and Actuators B: Chemical - Volume 107, Issue 1, 27 May 2005, Pages 82-87
نویسندگان
S. Godoy, B. Leca-Bouvier, P. Boullanger, L.J. Blum, A.P. Girard-Egrot,