کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10410446 | 894437 | 2005 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A DNA-detection platform with integrated photodiodes on a silicon chip
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In this paper, we had proposed a silicon integrated circuit (IC) compatible DNA-detection platform using photodiode and ordinary optical illumination to detect DNA hybridization events occurred on the chip surface. The method utilizes gold nanoparticle labeling and silver enhancement to generate opaque surface at area with target DNA hybridization. A photodiode fabricated underneath the supporting surface can then be used to detect the light intensity changes, before and after the silver enhancement. With this technique, we have successfully discriminated single base-mismatched DNA from matched DNA sequence, and the detection limit could be as low as 10Â pM. By exploiting the advanced modern IC manufacturing technology and the well-established electrochemical immobilization of DNA, this approach has the potential to be used to construct a portable high-density DNA nanoarray system with fully electronic readout.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 106, Issue 1, 29 April 2005, Pages 378-382
Journal: Sensors and Actuators B: Chemical - Volume 106, Issue 1, 29 April 2005, Pages 378-382
نویسندگان
Jiong Li, Chen Xu, Zhikuan Zhang, Yijin Wang, Huajun Peng, Zuhong Lu, Mansun Chan,