کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10411073 | 894540 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Quartz crystal microbalance assay for determination of plasma vitellogenin
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موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Anti-vitellogenin antibody was immobilized on a quartz crystal oscillator and applied to a quartz crystal microbalance (QCM) for detection of plasma vitellogenin (Vtg) of fish. The QCM exhibited a significant decrease in frequency responding to Vtg addition. The association constant K for the antigen-antibody reaction was determined to be 7 à 107 Mâ1. The QCM was also sensitive to plasma from Koi carp (Cyprinus carpio), of which Vtg synthesis had been stimulated by injection of estradiol-17β. Although nonspecific response to components in the plasma was observed, it was suppressed by pretreatment of the QCM device with bovine serum albumin.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 105, Issue 2, 28 March 2005, Pages 473-478
Journal: Sensors and Actuators B: Chemical - Volume 105, Issue 2, 28 March 2005, Pages 473-478
نویسندگان
Kenji Oshima, Hiromichi Nakajima, Shouji Takahashi, Yoshio Kera, Masato Shimomura, Shinnosuke Miyauchi,