کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10411085 | 894540 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Visualization of DNA hybridization on gold thin film by utilizing the resistance effect of DNA monolayer
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We propose a novel detection method for a duplex formation of DNA on a solid substrate. In this method, DNA monolayers are prepared on a gold thin film, and then the substrate is treated by an etching process. Since the DNA monolayer can act as a resist layer, the thickness of the gold film in the regions covered by DNA monolayers is larger than that in the region without the monolayer. Furthermore, the difference in the resistance effect of ss- and ds-DNA monolayers induces the difference in the thickness of the gold thin film after the etching. The resistance effect of the ss-probe DNA monolayer increases as a result of the formation of a duplex with the target DNA. The sequence-specific hybridization adsorption of the target DNA can be detected visually by measuring the light transmittance of the gold film, or with the naked eye. Using this method, we succeeded in detecting two base pair mismatches in a 30-base pair nucleotide.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 105, Issue 2, 28 March 2005, Pages 556-561
Journal: Sensors and Actuators B: Chemical - Volume 105, Issue 2, 28 March 2005, Pages 556-561
نویسندگان
Takao Masuda, Akira Yamaguchi, Masayuki Hayashida, Fumika Asari-Oi, Shigeki Matsuo, Hiroaki Misawa,