کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10429171 | 909698 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Rapid Cloning and Expression of Glutaryl-7-Aminocephalosporanic Acid Acylase Genes from Soil Samples
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موضوعات مرتبط
مهندسی و علوم پایه
سایر رشته های مهندسی
مهندسی (عمومی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A polymerase chain reaction (PCR)-based strategy was developed to rapidly obtain the gene encoding for an industrially important enzyme, glutaryl-7-aminocephalosporanic acid (GL-7-ACA) acylase. Different soil samples were cultured with a Pseudomonas selective medium to enrich specific microorganisms, and then the genomic DNA was extracted to serve as PCR templates. PCR primers for GL-7-ACA acylase gene amplification were designed on the basis of bioinformatics searches and analyses. The method was used to successfully amplify three GL-7-ACA acylase genes from different soil samples. The GL-7-ACA acylase genes were then cloned and overexpressed in Escherichia coli with a relatively high level of 266 unit·Lâ1.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Tsinghua Science & Technology - Volume 10, Issue 5, October 2005, Pages 529-534
Journal: Tsinghua Science & Technology - Volume 10, Issue 5, October 2005, Pages 529-534
نویسندگان
Luo (ç½ æ), Yu (äºæ
§æ), Li (æ 强), Shen (æ²å¿ è),