کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10431119 | 910196 | 2016 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Printable low-cost, sustained and dynamic cell stretching apparatus
ترجمه فارسی عنوان
دستگاه کشش سلولی کم هزینه، پایدار و پویا چاپ شده
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کلمات کلیدی
مکانیکولوژی، مکانیک انتقال، بیوراکتور، چاپ سه بعدی،
موضوعات مرتبط
مهندسی و علوم پایه
سایر رشته های مهندسی
مهندسی پزشکی
چکیده انگلیسی
Deformations that are applied on body tissues during daily activities, as a result of morbid conditions, or during various medical treatments, affect cell viability and biological function. Such mechanobiological phenomena are often studied in vitro, in monolayer cultures. To facilitate such studies cost effectively, we have developed a novel, printable cell stretching apparatus. The apparatus is used to apply tensile strains on cells cultured on elastic, stretchable substrata, either by sustained or by dynamic-cyclic application. Most of the apparatus parts are three-dimensionally printed (excluding motors), and stretching is automatically performed by two direct current geared motors that are controlled by a programmable microcontroller platform. To demonstrate functionality of this novel printable device, which can be produced in multiple copies in research labs at a cost of under 100 US$ per unit, including motors and controller, we performed cell culture studies monitored by fluorescence microscopy. Specifically, we have applied sustained and cyclic, radial stretching at large strains to NIH3T3 mouse fibroblasts, and have demonstrated that cell viability, adhesion and morphology were maintained following stretching. Our apparatus is designed to be low-cost, rapidly manufactured at a university or small-company setting, and simple to use and control, where its flexible, versatile design allows users to experimentally induce different stretching regimes with varying amplitudes and frequencies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biomechanics - Volume 49, Issue 8, 24 May 2016, Pages 1336-1339
Journal: Journal of Biomechanics - Volume 49, Issue 8, 24 May 2016, Pages 1336-1339
نویسندگان
Samer Toume, Amit Gefen, Daphne Weihs,