کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10431120 | 910196 | 2016 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Quantification of angiogenic sprouting under different growth factors in a microfluidic platform
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کلمات کلیدی
ECMPDGF-BBMicrofluidicsBMP2TGFβ1EGM-2Angiogenesis - آنژیوژنزSprouting - جوانه زدنEndothelial cells - سلولهای اندوتلیالThree-dimensions - سه بعدیGrowth factors - فاکتور رشدVascular endothelial growth factor - فاکتور رشد اندوتلیال عروقیVascular Endothelial Growth Factor (VEGF) - فاکتور رشد اندوتلیال عروقی (VEGF)Extracellular matrix - ماتریکس خارج سلولیCollective cell migration - مهاجرت سلولی جمعی
موضوعات مرتبط
مهندسی و علوم پایه
سایر رشته های مهندسی
مهندسی پزشکی
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چکیده انگلیسی
Angiogenesis, as example of collective migration of endothelial cells (ECs), is the main dynamic process that culminates in sprout formation from existing vessels. After tissue injury, the vascularity is interrupted, triggering the regeneration process and the release of different growth factors (GFs). The main aim of this work is to quantify the effect of specific GFs during the initial stage of sprout formation, namely: VEGF, PDGF-BB, TGFβ and BMP-2, all of them involved in regenerative processes. For this purpose, we designed a novel algorithm implemented in Matlab to quantify the advance of the EC monolayer over time and the sprout structure in 3D. Our results show that VEGF is the main regulatory GF on angiogenesis processes, producing longer sprouts with higher frequency. However, the chemoattractant effect of VEGF depends on its concentration and its spatiotemporal location, having a critical impact on the sprout time evolution. PDGF-BB (namely as PDGF) has a global negative effect on both the number and length of sprouts. TGFβ enhances sprout length at earlier times, although its effect gradually disappears over time. Finally, BMP-2 produces, overall, less number and shorter sprouts, but was the only GF with a positive evolution at longer times, producing fewer but longer sprouts.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biomechanics - Volume 49, Issue 8, 24 May 2016, Pages 1340-1346
Journal: Journal of Biomechanics - Volume 49, Issue 8, 24 May 2016, Pages 1340-1346
نویسندگان
Cristina Del Amo, Carlos Borau, Raquel Gutiérrez, Jesús AsÃn, José Manuel GarcÃa-Aznar,