کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10431499 | 910215 | 2015 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Dynamic hydrostatic pressure enhances differentially the chondrogenesis of meniscal cells from the inner and outer zone
ترجمه فارسی عنوان
فشار هیدرواستاتیک پویا به طور متقارن باعث افزایش جذب سلول های منسکی از ناحیه درونی و بیرونی می شود
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کلمات کلیدی
منیزوک، فیبروکاندروسیت ها، فشار هیدرواستاتیک پویا، مکانیکولوژی،
موضوعات مرتبط
مهندسی و علوم پایه
سایر رشته های مهندسی
مهندسی پزشکی
چکیده انگلیسی
This study analyses the influence of dynamic hydrostatic pressure on chondrogenesis of human meniscus-derived fibrochondrocytes and explores the differences in chondrogenic differentiation under loading conditions between cells derived from the avascular inner zone and vascularized outer region of the meniscus. Aggregates of human fibrochondrocytes with cell origin from the inner region or with cell origin from the outer region were generated. From the two groups of either cell origin, aggregates were treated with dynamic hydrostatic pressure (1Â Hz for 4Â h; 0.55-5.03Â MPa, cyclic sinusoidal) from day 1 to day 7. The other aggregates served as unloaded controls. At day 0, 7, 14 and 21 aggregates were harvested for evaluation including histology, immunostaining and ELISA analysis for glycosaminoglycan (GAG) and collagen II. Loaded aggregates were found to be macroscopically larger and revealed immunohistochemically enhanced chondrogenesis compared to the corresponding controls. Loaded or non-loaded meniscal cells from the outer zone showed a higher potential and earlier onset of chondrogenesis compared to the cells from the inner part of the meniscus. This study suggests that intrinsic factors like cell properties in the different areas of the meniscus and their reaction on mechanical load might play important roles in designing Tissue Engineering strategies for meniscal repair in vivo.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biomechanics - Volume 48, Issue 8, 1 June 2015, Pages 1479-1484
Journal: Journal of Biomechanics - Volume 48, Issue 8, 1 June 2015, Pages 1479-1484
نویسندگان
J. Zellner, M. Mueller, Y. Xin, W. Krutsch, A. Brandl, R. Kujat, M. Nerlich, P. Angele,