کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10432010 910233 2014 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Thyroid-stimulating hormone maintains bone mass and strength by suppressing osteoclast differentiation
ترجمه فارسی عنوان
هورمون تحریک کننده تیروئید توده استخوانی و قدرت را با سرکوب تمایز استئوکلاست حفظ می کند
موضوعات مرتبط
مهندسی و علوم پایه سایر رشته های مهندسی مهندسی پزشکی
چکیده انگلیسی
It has been suggested that pituitary hormone might be associated with bone metabolism. To investigate the role of thyroid-stimulating hormone (TSH) in bone metabolism, we designed the present study as follows. After weaning, TSH receptor (TSHR) null mice (Tshr−/−) were randomly divided into a thyroxine treatment group (n=10) or non-treatment group (n=10); the treatment group received a dose of desiccated thyroid extract at 100 ppm daily for 5 weeks. Age-matched wild-type (Tshr+/+, n=10) and heterozygote mice (Tshr+/−, n=10) served as controls. After 5 weeks, the animals were sacrificed, and the femurs were collected for histomorphometrical and biomechanical analyses. In addition, the effect of TSH on osteoclastogenesis was examined in the RAW264.7 osteoclast cell line. We found that compared with Tshr+/+ mice, Tshr−/− and Tshr+/− mice had lower bone strength. The histomorphometric results showed that trabecular bone volume, osteoid surface, osteoid thickness and osteoblast surface were significantly decreased, whereas the osteoclast surface was significantly increased in both Tshr−/− and Tshr+/− mice compared with Tshr+/+ mice. Bone resorption and formation in Tshr−/− mice were further enhanced by thyroxine replacement. bTSH inhibited osteoclast differentiation in vitro, as demonstrated by reduced development of TRAP-positive cells and down-regulation of differentiation markers, including tartrate-resistant acid phosphatase, matrix metallo-proteinase-9 and cathepsin K in RAW264.7 cells. Our results confirm that TSH increased bone volume and improved bone microarchitecture and strength at least partly by inhibiting osteoclastogenesis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biomechanics - Volume 47, Issue 6, 11 April 2014, Pages 1307-1314
نویسندگان
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