کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10432486 | 910248 | 2013 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Collagen abundance in mechanically stimulated osteoblast cultures using near infrared microscopy
ترجمه فارسی عنوان
فراوانی کلاژن در کشت های استئوبلاستی مکانیکی تحریک شده با استفاده از میکروسکوپ نزدیک مادون قرمز
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
سایر رشته های مهندسی
مهندسی پزشکی
چکیده انگلیسی
Collagen abundance in osteoblast cell cultures was determined using near infrared microscopy with chemical imaging (NIR-CI) with and without mechanical stimulation of the the cells. MC3T3-E1 mouse osteoblast cells seeded on a polycarbonate substrate were mechanically stimulated using static loads of 13.5Â N, 27Â N and 40Â N applied to the substrates during 2, 4, 6 and 8 days of incubation. Results show that the cells increased their collagen production with 13.5Â N and 27Â N loads when compared to the control sample with a 27Â N load resulting in a noteworthy increase (109%) in collagen production. The 40Â N load on the other hand, resulted in an initial decrease in the collagen expression in the extracellular matrix, possibly as a result of cell death or inhibition of the protein secretion process followed by an increase in collagen after cell recovery and proliferation. Qualitative confirmation of these results was performed using confocal microscopy.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Biomechanics - Volume 46, Issue 14, 27 September 2013, Pages 2442-2450
Journal: Journal of Biomechanics - Volume 46, Issue 14, 27 September 2013, Pages 2442-2450
نویسندگان
Jairo Boada-Lopez, Idaris DeJesus-Maldonado, Jackeline Jerez, Rodolfo Romañach, Nanette Diffoot-Carlo, Paul Sundaram,