کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10496 687 2008 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Angiomyogenesis using liposome based vascular endothelial growth factor-165 transfection with skeletal myoblast for cardiac repair
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Angiomyogenesis using liposome based vascular endothelial growth factor-165 transfection with skeletal myoblast for cardiac repair
چکیده انگلیسی

We aim to investigate the feasibility and efficacy of cholesterol (Chol) + DOTAP liposome (CD liposome) based human vascular endothelial growth factor-165 (hVEGF165) gene transfer into human skeletal myoblasts (hSkM) for cardiac repair. The feasibility and efficacy of CD liposome for gene transfer with hSkM was characterized using plasmid carrying enhanced green fluorescent protein (pEGFP). Based on the optimized transfection procedure, hSkM were transfected with CD lipoplexes carrying plasmid-hVEGF165 (CD–phVEGF165). The genetically modified hSkM were transplanted into rat heart model of acute myocardial infarction. Flow cytometry revealed that about 7.99% hSkM could be transfected with pEGFP. Based on the optimized transfection condition, transfected hSkM expressed hVEGF165 up to day-18 (1.7 ± 0.1 ng/ml) with peak at day-2 (13.1 ± 0.52 ng/ml) with >85% cell viability. Animal studies revealed that reduced apoptosis, improved angiogenesis with blood flow in group-3 animal's heart were achieved as compared to group-1 and 2. Ejection fraction was best recovered in group-3 animals. The study demonstrates that though gene transfection efficiency using CD liposome mediated hVEGF165 gene transfer with hSkM was low; hVEGF165 gene expression efficiency was sufficient to induce neovascularization, improve blood flow and injured heart function.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 29, Issue 13, May 2008, Pages 2125–2137
نویسندگان
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