کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10513 688 2009 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Bienzyme functionalized three-layer composite magnetic nanoparticles for electrochemical immunosensors
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Bienzyme functionalized three-layer composite magnetic nanoparticles for electrochemical immunosensors
چکیده انگلیسی

The preparation, characterization and application of a three-layer magnetic nanoparticle composed of an Fe3O4 magnetic core, a Prussian Blue (PB) interlayer and a gold shell (it can be abbreviated as Au–PB–Fe3O4) for an ultrasensitive and reproducible electrochemical immunosensing fabrication were described for the first time in this work. With the employment of the Au–PB–Fe3O4 nanoparticle, a new signal amplification strategy was developed based on bienzyme (horseradish peroxidase and glucose oxidase) functionalized Au–PB–Fe3O4 nanoparticles for an electrochemical immunosensing fabrication by using Carcinoembryonic antigen (CEA) and α-fetoprotein (AFP) as model systems, respectively. The experiment results show that the multilabeled Au–PB–Fe3O4 nanoparticles exhibit satisfying redox electrochemical activity and high enzyme catalysis activity, which predetermine their utility in high sensitivity antibody detection schemes. Furthermore, this immunosensor could be regenerated by simply using an external magnetic field which ensured a reproducible immunosensor with high sensitivity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 30, Issue 12, April 2009, Pages 2284–2290
نویسندگان
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