کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10532553 | 961609 | 2013 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Measuring the antioxidant capacity of blood plasma using potentiometry
ترجمه فارسی عنوان
اندازه گیری ظرفیت آنتی اکسیدانی پلاسمای خون با استفاده از پتانسیومتری
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کلمات کلیدی
پتانسیومتری، ظرفیت آنتی اکسیدان کل، پلاسما، ورزش کامل،
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
The use of potentiometry to measure plasma antioxidant capacity to contribute to oxidative stress evaluation is presented. In this assay, plasma (n = 60) diluted (0.3 to 1 ml) in phosphate buffer, pH 7.4, NaCl 9%, was submitted to potentiometry. A platinum wire was the working electrode and saturated calomel the reference. The results are presented as the difference between sample and buffer potential (ÎE). ÎE presented a good inverse correlation with added increasing concentrations of ascorbate (2.5â75 μmol/L; R = â0.99), urate (9.0â150 μmol/L; R = â0.99), and bilirubin (0.78â13 μmol/L; R = â0.99). Increase in the antioxidant capacity decreased ÎE. Depletion of the antioxidant capacity by tert-butylhydroperoxide (6.5â50 μmol/L) presented a direct correlation (0.97) with ÎE. Furthermore, ÎE presented an inverse correlation (R = â0.99) with increased antioxidant capacity of plasma (FRAP) induced by the addition of ascorbate (2.5â75 μmol/L). The response of the potentiometric method proved be adequate for measuring the plasma antioxidant depletion induced by acute exhaustive exercise in rats (control, n = 15; exercised, n = 15). This exercise decreased the concentration of urate (p < 0.05), decreased FRAP (p < 0.5), increased TBARS (p < 0.5), and decreased the potentiometer sensor response (p = 6.5 Ã 10â3). These results demonstrate the adequacy of potentiometry for evaluating the antioxidant capacity of blood plasma samples.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 441, Issue 2, 15 October 2013, Pages 109-114
Journal: Analytical Biochemistry - Volume 441, Issue 2, 15 October 2013, Pages 109-114
نویسندگان
L.S. Tessutti, D.V. Macedo, L.T. Kubota, A.A. Alves,