کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10532644 | 961686 | 2013 | 36 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Magnetic nanoparticle-based immunosensor for electrochemical detection of hepatitis B surface antigen
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
An electrochemical immunosensor was developed for the detection of hepatitis B surface antigen (HBsAg). The biotinylated hepatitis B surface antibody was immobilized on streptavidin magnetic nanoparticles and used for targeting the HBsAg. By the addition of horseradish peroxidase conjugated with secondary antibody (HRP-HBsAb), a sandwich-type immunoassay format was formed. Aminophenol as substrate for conjugated HRP was enzymatically changed into 3-aminophenoxazone (3-APZ). This electroactive enzymatic production (3-APZ) was transferred into an electrochemical cell and monitored by cyclic voltammetry. Under optimal conditions, the cathodic current response of 3-APZ, which was proportional to the HBsAg concentration, was measured by a glassy carbon electrode. The immunosensor response was linear toward HBsAg in the concentration range from 0.001 to 0.015Â ng/ml with a detection limit of 0.9Â pg/ml at a signal/noise ratio of 3.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 441, Issue 1, 1 October 2013, Pages 1-7
Journal: Analytical Biochemistry - Volume 441, Issue 1, 1 October 2013, Pages 1-7
نویسندگان
Sara Nourani, Hedayatollah Ghourchian, Seyed Mehdi Boutorabi,