کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10532672 | 961689 | 2011 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Study of interactions between DNA and aflatoxin B1 using electrochemical and fluorescence methods
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In this study, a carbon paste electrode modified with N-butylpyridinium hexafluorophosphate (BPPF6) ionic liquid and DNA was introduced as an electrochemical biosensor to study the interaction between DNA and aflatoxin B1 molecules. For this purpose, variations in oxidation peak current of guanine in various concentrations of aflatoxin B1 were measured by using the differential pulse voltammetry (DPV) method. According to this study, the binding constant of DNA-aflatoxin B1 was found to be 3.5Â ÃÂ 106Â Mâ1. This modified electrode was also used for determination of low concentrations of aflatoxin B1 by using differential pulse voltammetry. A linear dynamic range from 8.00Â ÃÂ 10â8 to 5.91Â ÃÂ 10â7Â M and a limit of detection of 2.00Â ÃÂ 10â8Â M resulted from DPV measurements. To confirm our results, a fluorescence study was also performed. It resulted in a binding constant of 2.8Â ÃÂ 106Â Mâ1, which is in good agreement with that obtained from electrochemical study.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 411, Issue 2, 15 April 2011, Pages 218-222
Journal: Analytical Biochemistry - Volume 411, Issue 2, 15 April 2011, Pages 218-222
نویسندگان
Mohammad Hossein Banitaba, Saied Saeed Hosseiny Davarani, Ali Mehdinia,