کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10532720 961707 2013 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Assessment of stable isotope incorporation into recombinant proteins
ترجمه فارسی عنوان
ارزیابی ترکیب ایزوتوپ پایدار در پروتئین های نوترکیب
کلمات کلیدی
برچسب زدن ایزوتوپ پایدار، طیف سنجی جرمی، ارزیابی کمی از ترکیب ایزوتوپ پایدار،
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی
Stable isotope labeling combined with mass spectrometry has been widely used in a diverse set of applications in the biochemistry and biomedical fields. When stable isotope-labeled proteins are produced via metabolic labeling of cell culture, a comprehensive assessment of the labeling pattern is imperative. In this study, we present a set of mass spectrometry-based bioanalytical tools developed for quantitatively tracing the levels of the stable isotopes incorporated into the recombinant proteins (monoclonal antibodies and Fc fusion proteins expressed in different host systems) that include total mass analysis, peptide mapping analysis, and amino acid analysis. We show that these three mass spectrometry-based analytical methods have distinctive advantages and limitations and that they are mutually complementary in evaluating the quality of stable isotope-labeled proteins. In addition, we show that the analytical techniques developed here are powerful tools to provide valuable insights into studying cell metabolism and performing flux analysis during cell culture.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 433, Issue 2, 15 February 2013, Pages 137-149
نویسندگان
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