کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10532770 | 961712 | 2010 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Polymerized and polyethylene glycol-conjugated hemoglobins: A globin-based calibration curve for dynamic light scattering analysis
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Polymerized and polyethylene glycol-conjugated hemoglobins: A globin-based calibration curve for dynamic light scattering analysis Polymerized and polyethylene glycol-conjugated hemoglobins: A globin-based calibration curve for dynamic light scattering analysis](/preview/png/10532770.png)
چکیده انگلیسی
Dynamic light scattering (DLS) is a technique capable of determining the hydrodynamic radius of proteins. From this parameter, a molecular weight can be assessed provided that an appropriate calibration curve is available. To this goal, a globin-based calibration curve was used to determine the polymerization state of a recombinant hemoglobin-based oxygen carrier and to assess the equivalent molecular weight of hemoglobins conjugated with polyethylene glycol molecules. The good agreement between DLS values and those obtained from gel filtration chromatography is a consequence of the high similarity in structure, shape, and density within the globin superfamily. Moreover, globins and heme proteins in general share similar spectroscopic properties, thereby reducing possible systematic errors associated with the absorption of the probe radiation by the chromophore.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 401, Issue 2, 15 June 2010, Pages 266-270
Journal: Analytical Biochemistry - Volume 401, Issue 2, 15 June 2010, Pages 266-270
نویسندگان
Serena Faggiano, Luca Ronda, Stefano Bruno, Hanna Jankevics, Andrea Mozzarelli,