کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10532846 | 961744 | 2011 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
5-Ethynyl-2â²-deoxycytidine as a new agent for DNA labeling: Detection of proliferating cells
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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![عکس صفحه اول مقاله: 5-Ethynyl-2â²-deoxycytidine as a new agent for DNA labeling: Detection of proliferating cells 5-Ethynyl-2â²-deoxycytidine as a new agent for DNA labeling: Detection of proliferating cells](/preview/png/10532846.png)
چکیده انگلیسی
The labeling of newly synthesized DNA in cells to identify cell proliferation is an important experimental technique. The most accurate methods incorporate [3H]thymidine or 5-bromo-2â²-deoxyruidine (BrdU) into dividing cells during S phase, which is subsequently detected by autoradiography or immunohistochemistry, directly measuring the newly synthesized DNA. Recently, a novel method was developed to detect DNA synthesis in proliferating cells based on a novel thymidine analog, 5-ethynyl-2â²-deoxyuridine (EdU). EdU is incorporated into DNA and subsequently detected with a fluorescent azide via “click” chemistry. This novel technique is highly sensitive and does not require DNA denaturation. However, it was also found that EdU exhibits time-dependent inhibition effects on cell growth. Therefore, here we report a novel deoxycytidine analog, 5-ethynyl-2â²-deoxycytidine (EdC), that can be used to detect DNA synthesis in vitro and in vivo at a similar sensitivity level compared with EdU. Furthermore, the EdC-induced cytotoxicity is much less than that of EdU when combined with thymidine. This will be a potential application for the long-term detection of proliferating cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 417, Issue 1, 1 October 2011, Pages 112-121
Journal: Analytical Biochemistry - Volume 417, Issue 1, 1 October 2011, Pages 112-121
نویسندگان
Dezhong Qu, Guoxing Wang, Zhe Wang, Li Zhou, Weilin Chi, Shujie Cong, Xiaoshuai Ren, Peizhou Liang, Biliang Zhang,