کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10533152 961851 2005 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Amperometric biosensor for determining human salivary phosphate
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Amperometric biosensor for determining human salivary phosphate
چکیده انگلیسی
An amperometric biosensor was constructed for analysis of human salivary phosphate without sample pretreatment. The biosensor was constructed by immobilizing pyruvate oxidase (PyOD) on a screen-printed electrode. The presence of phosphate in the sample causes the enzymatic generation of hydrogen peroxide (H2O2), which was monitored by a potentiostat and was in proportion to the concentration of human salivary phosphate. The sensor shows response within 2 s after the addition of standard solution or sample and has a short recovery time (2 min). The time required for one measurement using this phosphate biosensor was 4 min, which was faster than the time required using a commercial phosphate testing kit (10 min). The sensor has a linear range from 7.5 to 625 μM phosphate with a detection limit of 3.6 μM. A total of 50 salivary samples were collected for the determination of phosphate. A good level of agreement (R2 = 0.9646) was found between a commercial phosphate testing kit and the phosphate sensor. This sensor maintained a high working stability (>85%) after 12 h operation and required only a simple operation procedure. The amperometric biosensor using PyOD is a simple and accurate tool for rapid determinations of human salivary phosphate, and it explores the application of biosensors in oral and dental research and diagnosis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 343, Issue 2, 15 August 2005, Pages 263-267
نویسندگان
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