کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10533208 961856 2005 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Electroimmobilization of proinsulin C-peptide to a quartz crystal microbalance sensor chip for protein affinity purification
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Electroimmobilization of proinsulin C-peptide to a quartz crystal microbalance sensor chip for protein affinity purification
چکیده انگلیسی
Proinsulin C-peptide was electroimmobilized to a quartz crystal microbalance sensor chip, localizing this low-pI peptide for covalent attachment to activated surface carboxyl groups. The resulting chip was used in a continuous flow biosensor to capture anti-C-peptide antibodies, which could subsequently be eluted in 5% formic acid between air bubbles for efficient recovery and mass spectrometric identification. The method is reproducible through repeated cycles, providing affinity purification of proteins under real-time monitoring of the binding and elution processes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 341, Issue 1, 1 June 2005, Pages 89-93
نویسندگان
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