کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10533267 | 961861 | 2005 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A colorimetric assay for the determination of 4-diphosphocytidyl-2-C-methyl-d-erythritol 4-phosphate synthase activity
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A new method for the determination of the activity of 4-diphosphocytidyl-2-C-methyl-d-erythritol 4-phosphate synthase, the enzyme catalyzing the third reaction of the 2-C-methyl-d-erythritol 4-phosphate pathway for biosynthesis of isoprenoids, is described. This is an end-point assay based on the transformation of inorganic pyrophosphate, one of the products of the reaction, to phosphate by using inorganic pyrophosphatase as auxiliary enzyme. The phosphate formed is reacted then with the dye malachite green to yield a colored product which can be determined spectrophotometrically. The method is easy to perform, sensitive, and robust and can be used in automated high-throughput screening analyses for the search of inhibitors of the enzyme.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 337, Issue 1, 1 February 2005, Pages 55-61
Journal: Analytical Biochemistry - Volume 337, Issue 1, 1 February 2005, Pages 55-61
نویسندگان
Cristobal Bernal, Celia Palacin, Albert Boronat, Santiago Imperial,