کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10533316 961866 2005 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Measurement of DNA methylation using stable isotope dilution and gas chromatography-mass spectrometry
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Measurement of DNA methylation using stable isotope dilution and gas chromatography-mass spectrometry
چکیده انگلیسی
A simple, highly selective, and sensitive method has been developed to quantify methylation of DNA extracted from human peripheral blood mononuclear cells. Assay has been performed at nucleobases level. Cytosine and 5-methylcytosine DNA content has been detected by gas chromatography-mass spectrometry using [2-13C]cytosine and [2-13C]5-methylcytosine as internal standards. The methylation level has been calculated as 5-methylcytosine/total cytosine ratio. The working range selected on calibration curve, obtained by evaluation of standards and matrix-added standards measurements, is suitable for 5 μg DNA analysis. In this range, healthy human DNA methylation percentage is within 5-6%.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 336, Issue 2, 15 January 2005, Pages 158-163
نویسندگان
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