کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10536256 | 962052 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Ion-selective enrichment of tyrosine-sulfated peptides from complex protein digests
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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![عکس صفحه اول مقاله: Ion-selective enrichment of tyrosine-sulfated peptides from complex protein digests Ion-selective enrichment of tyrosine-sulfated peptides from complex protein digests](/preview/png/10536256.png)
چکیده انگلیسی
We have developed a novel procedure for concentrating sulfated peptides, as a front end to mass spectrometric analysis, based on ion-selective interaction of sulfate ions with anion exchangers. Ions with a higher charge and smaller solvated ion radius, such as sulfate ions, have higher retention in an ion exchanger due to their greater degree of coulombic interactions. We tested the effectiveness of this approach for enrichment and identification of sulfated peptides using a tryptic digest of bovine serum albumin spiked with model sulfated peptide (molar ratio 20:1) and using a tryptic digest of bovine fibrinogen. Sulfated peptides are identified by mass spectrometry in which both the molecular ion and its specific fragment ion produced by facile loss of SO3 are detected. In both experiments, sulfated peptides were strongly retained on the anion exchanger and were eluted by higher concentrations of competing ion with minimal contamination of nonsulfated peptides. Using this procedure, we determined that the 13-amino acid C-terminal peptide of the minor γâ²-chain of bovine fibrinogen contains sulfated tyrosine.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 346, Issue 1, 1 November 2005, Pages 124-131
Journal: Analytical Biochemistry - Volume 346, Issue 1, 1 November 2005, Pages 124-131
نویسندگان
Yukari Amano, Hidefumi Shinohara, Youji Sakagami, Yoshikatsu Matsubayashi,