کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10602 693 2009 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The regulation of the gap junction of human mesenchymal stem cells through the internalization of quantum dots
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
The regulation of the gap junction of human mesenchymal stem cells through the internalization of quantum dots
چکیده انگلیسی

The delivery mechanism of CdSe/ZnS quantum dots (QDs) into cells was previously found to critically determine the biocompatibility of QDs to human adult mesenchymal stem cells, but the associated mechanism remained unknown. The present study tried to establish a link between the above phenomenon and the change in gap junction upon QD internalization. By comparing Pep-1- and PolyFect-mediated QD internalizations, the connexin 43 (Cx43)-mediated gap junction intercellular communication (GJIC) of human adipose-derived adult stem cells was investigated in monolayer and in three-dimensional (3D) culture (alginate hollow spheres). The latter system offered cells more mobility, which was more similar as in vivo. The results showed that Pep-1-coated QDs, which escaped from the endo-/lysosome degradation, could activate the F-actin assembly and the ERK-dependent phosphorylation of Cx43. The consequence was a reduction in Cx43-mediated GJIC. When the cells were grown in high density 3D alginate hollow spheres instead of in monolayer, the decrease of GJIC caused by the QD internalization was restored. These results indicated that the adaptability in QDs-mediated regulation of GJIC with different delivery coatings depended on the culture systems. The study also suggested that the regulation of gap junction may play a key role in QD cytotoxicity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 30, Issue 10, April 2009, Pages 1937–1946
نویسندگان
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