کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10639970 | 995862 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Two-dimensional differential adherence and alignment of C2C12 myoblasts
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موضوعات مرتبط
مهندسی و علوم پایه
مهندسی مواد
مواد الکترونیکی، نوری و مغناطیسی
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
Surface microstructure is a critical parameter for scaffolds used in skeletal muscle tissue engineering. We have developed micromachined surfaces using matrix assisted pulsed laser evaporation-direct write (MAPLE-DW) that demonstrate differential adherence of C2C12 myoblast cells. The 60-400 μm diameter channels were micromachined onto 2% agarose surfaces using an ArF excimer laser and lined with Matrigel® basement membrane matrix solution. Suspensions containing C2C12 myoblast cells were then placed on the surface of these micromachined channels. The C2C12 myoblast cells aligned themselves parallel to the 60-150 μm channels. Live/dead assays over 72 h demonstrated that cell number, cell size, and number of nuclei per cell increased within these channels. In addition, some of the myoblasts fused and differentiated into multinucleated myotubes. These results provide the basis for the development of direct-write scaffolds for skeletal muscle tissue engineering.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Materials Science and Engineering: B - Volume 123, Issue 3, 25 November 2005, Pages 242-247
Journal: Materials Science and Engineering: B - Volume 123, Issue 3, 25 November 2005, Pages 242-247
نویسندگان
T.M. Patz, A. Doraiswamy, R.J. Narayan, R. Modi, D.B. Chrisey,