Live cell calcium imaging at the single ion hit facility of GSI

To study the fast intracellular calcium response after ion irradiation in living mammalian cells, a live cell calcium imaging set-up was constructed at the targeted cell irradiation facility at GSI. This work introduces the live cell calcium imaging system, shows its performance, an example of the ratio-metric calcium measurement and its application to on-line study calcium response to targeted ion irradiation in human cells.