کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10674902 1010552 2005 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Mutagenic effect of a keV range N+ beam on mammalian cells
موضوعات مرتبط
مهندسی و علوم پایه مهندسی مواد سطوح، پوشش‌ها و فیلم‌ها
پیش نمایش صفحه اول مقاله
Mutagenic effect of a keV range N+ beam on mammalian cells
چکیده انگلیسی
The radiobiological effects of a keV (5-20 keV) range nitrogen ion (N+) beam on mammalian cells were studied, particularly with regard to the induction of mutation in the cell genome. The experiment demonstrated that the 20 keV N+ beam, which resulted in cell death to a certain extent, induced a 2-3 fold increase in the mutation rates at the CD59 gene locus of the mammalian AL cells as compared to the control. Within certain fluence ranges (0-6 × 1014 N+/cm2), the cell survival displayed a down-up-down pattern which is similar to the phenomenon known as 'hyper-radiosensitivity' manifested under low-dose irradiation; the CD59 mutation rate firstly showed a gradual rise up to a 3-fold increment above the background level as the ion fluence went up to 4 × 1014 N+/cm2, after this peak point however, a downtrend appeared though the ion fluence increased further. It was also observed that the fraction of CD59 mutation bears no proportional relation to ion energy in further experiments of mutation induction by N+ beams with the incident energies of 5, 10, 15 and 20 keV at the same fluence of 3 × 1014 N+/cm2. Analyses of the deletion patterns of chromosome 11 in CD59− mutants induced by 5-20 keV N+ beams showed that these ions did not result in large-size chromosome deletions in this mammalian cell system. A preliminary discussion, suggesting that the mutagenic effect of such low-energy ion influx on mammalian cells could result from multiple processes involving direct collision of particles with cellular DNA, and cascade atomic and molecular reactions due to plentiful primary and secondary particles, was also presented. The study provided the first glimpse into the roles low-energy ions may play in inducing mutagenesis in mammalian cells, and results will be of much value in helping people to understand the contribution of low-energy ions to radiological effects of various ionising radiations.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Nuclear Instruments and Methods in Physics Research Section B: Beam Interactions with Materials and Atoms - Volume 234, Issue 4, July 2005, Pages 477-486
نویسندگان
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