Bio-, nano-technology for cellular biosensing

Next one is a genetical construction of postsynapse model cell for an evaluation of extracellular neural transmitter. Postsynaptic function is presented by channel gate receptor on postsynaptic membrane. In the present study, cloned glutamic acid channel gate receptor (GluR-D) gene is transformed into insect cell Sf-9. Sf-9 is easy to cultivate and is able to express GluR-D on its cell surface as same as postsynaptic membrane. Its function can be monitored by outer cell potential. Both of tactics is successfully performed and is applicatable cultured cell-based assay on pharmaceutical screening and chemical safety proofing.