کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10747111 | 1048893 | 2015 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Changes of spontaneous parthenogenetic activation and development potential of golden hamster oocytes during the aging process
ترجمه فارسی عنوان
تغییرات فعالیت پاراتونوژنیک خودبهخود و پتانسیل رشد تخمک های هامستر طلایی در طول فرآیند پیری
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کلمات کلیدی
HDACs6-DMAPCOCsCGSAPFCCS6-Dimethylaminopurine - 6-دی متیل آمینوپورینHCG - اچ سی جیIntracytoplasmic injection - تزریق داخل سیتوپلاسمیکcumulus cells - سلول های کومولوسIn vitro fertilization - لقاح در خارج از بدن یا آیویافIVF - لقاح مصنوعی cumulus–oocyte complexes - مجتمع های کومولوس-اووسیتICSI - میکرواینجکشنMII - هزارhistone deacetylases - هیستون deacetylaseshistone acetyltransferases - هیستون استیل ترانسفرازHATs - کلاهcortical granules - گرانول های قارچیhuman chorionic gonadotropin - گونادوتروپین کوریونی انسان
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
چکیده انگلیسی
The golden hamster is an excellent animal experimental model for oocyte research. The hamster oocytes are very useful in clinical examination of human spermatozoan activity. Non-fertile oocytes can lead to time-dependent processes of aging, which will affect the results of human spermatozoa examination. As a consequence there is a need to investigate the aging and anti-aging processes of golden hamster oocytes. In order to study the aging processes and parthenogenetic activation of golden hamster oocytes, in vivo oocytes, oocytes cultured with or without cumulus cells, and oocytes treated with Trichostatin A (TSA) or caffeine were collected and investigated. We found that: (1) spontaneous parthenogenetic activation, developmental potential (cleavage rate), and zona pellucida (ZP) hardening undergo age-dependent changes in in vivo, in vitro, and after TSA or caffeine treatment; (2) in vivo, oocytes became spontaneously parthenogenetic 25Â h post-hCG treatment; (3) in vitro, cumulus cells did not significantly increase the parthenogenetic activation rate of cultured hamster oocytes; and (4) TSA or caffeine could delay spontaneous oocyte parthenogenetic activation and the aging processes by at least 5Â h, but also accelerated the hardening of the ZP. These results define the conditions for the aging and anti-aging processes in golden hamster oocytes. TSA and caffeine play roles in controlling spontaneous activation, which could facilitate the storage and use of golden hamster oocytes for studying processes relevant to human reproduction.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Acta Histochemica - Volume 117, Issue 1, January 2015, Pages 104-110
Journal: Acta Histochemica - Volume 117, Issue 1, January 2015, Pages 104-110
نویسندگان
Han Jiang, Ce Wang, Jiyu Guan, Lingyan Wang, Ziyi Li,