کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10747306 | 1048935 | 2011 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Immunohistochemical localization of CD31, NOTCH1 and JAGGED1 proteins in experimentally induced polycystic ovaries of immature rats
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کلمات کلیدی
CD31Cystic follicleNotch1Jagged1FFPE - MEPcorpora lutea - اجسام زردOvary - تخمدانformalin fixed paraffin embedded - فرمالین ثابت پارافین تعبیه شده استAntral follicle - فولیکول آنترالPrimary follicle - فولیکول اولیهPreantral follicle - فولیکول پیش قلبیRats - موش صحراییPAF - نیروی هوایی پاکستانHematoxylin and Eosin - هماتوکسیلین و ائوزینfollicle-stimulating hormone - هورمون تحریک کننده فولیکولluteinizing hormone - هورمون جسم زردFSH - هورمون محرکه فولیکولی Hyperandrogenemia - هیپرآندروژنیک
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We analyzed histomorphometrical changes and blood vessel immunohistochemical staining of CD31, NOTCH1 and JAGGED1 in induced polycystic ovaries of immature female Wistar rats, as well as serum hormone levels. The rats were randomly divided into control (n=18) and treated (n=18) groups. Treated animals received intramuscularly testosteronenantat weekly (0.1Â mg/g). Controls received the same amount of ricinus oil. Rats were weighed daily. Control and treated subgroups (6 rats per subgroup) were subsequently sacrificed after 21, 28 and 35 days of treatment. In ovaries of treated rats we found large cystic follicles, thick stromal tissue, many atretic preantral follicles, no ovulation and a thinner granulosa cell layer. CD31 stained blood vessels in the theca layer were reduced, with reduced JAGGED1 and NOTCH1 immunostaining. In controls, preantral and antral follicles were larger than in the treated group. Treated animals showed statistically significant lower progesterone and higher testosterone levels. They gained more weight than controls. Reduced immunostaining for NOTCH1 and JAGGED1 of reduced blood vessels of the theca layer was found in all stages of folliculogenesis with a distinct reduction in cystic and atretic follicles. Our results provide evidence of intrinsic abnormality during all stages of folliculogenesis in polycystic ovaries and this may result from crosstalk between circulating gonadotropins and follicular angiogenesis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Acta Histochemica - Volume 113, Issue 3, May 2011, Pages 262-269
Journal: Acta Histochemica - Volume 113, Issue 3, May 2011, Pages 262-269
نویسندگان
Anita Skrtic, Lea Sokolic, Ana Borovecki, Jagoda Rosa, Vanja Fenzl,