کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10821824 | 1061547 | 2005 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Compensatory proteolytic responses to dietary proteinase inhibitors in the red flour beetle, Tribolium castaneum (Coleoptera: Tenebrionidae)
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کلمات کلیدی
SDSPAGESTIE-64BAPNAn-α-benzoyl-dl-arginine-p-nitroanilideEDTA - اتیلن دی آمین تترا استیک اسید polyacrylamide gel electrophoresis - الکتروفورز ژل پلی آکریل آمیدsodium dodecyl sulfate - سدیم دودسیل سولفاتProteinase inhibitors - مهار کننده های پروتئینازInsect digestion - هضم حشرهTribolium castaneum - کاکتوس Tribolium
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Increasing levels of inhibitors that target cysteine and/or serine proteinases were fed to Tribolium castaneum larvae, and the properties of digestive proteinases were compared in vitro. Cysteine proteinases were the major digestive proteinase class in control larvae, and serine proteinase activity was minor. Dietary serine proteinase inhibitors had minimal effects on either the developmental time or proteolytic activity of T. castaneum larvae. However, when larvae ingested cysteine proteinase inhibitors, there was a dramatic shift from primarily cysteine proteinases to serine proteinases in the proteinase profile of the midgut. Moreover, a combination of cysteine and serine proteinase inhibitors in the diet prevented this shift from cysteine proteinase-based digestion to serine proteinase-based digestion, and there was a corresponding substantial retardation in growth. These data suggest that the synergistic inhibitory effect of a combination of cysteine and serine proteinase inhibitors in the diet of T. castaneum larvae on midgut proteolytic activity and beetle developmental time is achieved through the prevention of the adaptive proteolytic response to overcome the activity of either type of inhibitor.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology - Volume 140, Issue 1, January 2005, Pages 53-58
Journal: Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology - Volume 140, Issue 1, January 2005, Pages 53-58
نویسندگان
B. Oppert, T.D. Morgan, K. Hartzer, K.J. Kramer,