کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10822021 | 1061561 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Effectiveness of recombinant soybean cysteine proteinase inhibitors against selected crop pests
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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چکیده انگلیسی
Three recombinant soybean cysteine proteinase inhibitors (rSCPIs), L1, R1 and N2, were assessed for their potential to inhibit the growth and development of three major agricultural crop pests known to utilize digestive cysteine proteinases: Western corn rootworm (Diabrotica virgifera virgifera, WCR), Colorado potato beetle (Leptinotarsa decemlineata, CPB) and cowpea weevil (Callosobruchus maculatus, CW). In vitro experiments showed that cysteine proteinase activities in the crude gut extracts of the WCR, CPB, and CW were inhibited to various degrees by the three rSCPIs. Of the three rSCPIs tested, N2 was most effective in inhibiting the crude gut extract of WCR, CPB, and CW (50% inhibition at 5Â ÃÂ 10â8, 5Â ÃÂ 10â8, and 3Â ÃÂ 10â7 M, respectively). The L1 was the least potent of the three CPIs tested, with 50% inhibition at 5Â ÃÂ 10â6 M of the crude gut extracts of WCR. Results of in vivo experiments conducted to assess the effect of the three rSCPIs on the vital growth parameters of WCR, CPB and CW were consistent with results of the in vitro experiments.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology - Volume 140, Issue 2, February 2005, Pages 227-235
Journal: Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology - Volume 140, Issue 2, February 2005, Pages 227-235
نویسندگان
S. Lalitha, R.E. Shade, L.L. Murdock, R.A. Bressan, P.M. Hasegawa, S.S. Nielsen,