Immunomodulatory roles and functional analysis of pre-B lymphocyte DT40 cells with the bursal-derived BSP-II treatment

► BSP-II induced strong humoral immune responses in immunized chicks. ► BSP-II induced DT40 cell viability at the reachable concentrations. ► Numerous genes expression were regulated after BSP-II treatment. ► Pathway analysis was performed to analysis BSP-II-regulated genes. ► RT-qPCR was used to confirm the microarray expression data.