Novel antimicrobial peptides isolated from the skin secretions of Hainan odorous frog, Odorrana hainanensis

► 7 antimicrobial peptides belonging to 5 families were identified from Odorrana hainanensis. ► Three antimicrobial peptide, temporin-HN1, temporin-HN2 and brevinin-1HN1 were purified from O. hainanensis. ► Each of these peptides was selected for the functional analysis, and displayed different antibacterial and hemolytic activity. ► Temporin-HN1, temporin-HN2 and brevinin-2HS2 display weak hemolytic activity in vitro.