Purification and biochemical characterization of antioxidant peptide from horse mackerel (Magalaspis cordyla) viscera protein

► Peptide with high antioxidant properties was isolated from the visceral waste of Magalaspis cordyla. ► Two steps of purification were executed using FPLC to obtain the active peptide. ► The sequence of purified peptide was determined as Ala-Cys-Phe-Leu (518.5 Da). ► Identified peptide proved better than natural antioxidant, α-tocopherol in PUFA protection.