Immunocytochemical analysis of putative allatostatin receptor (DAR-2) distribution in the CNS of larval Drosophila melanogaster

Allatostatins (ASTs) are a family of neuropeptides that inhibit the biosynthesis of juvenile hormone in cockroaches and related insects, but not in flies. Two receptors for allatostatins, DAR-1 and DAR-2, with sequence similarity to mammalian galanin receptors have previously been cloned in Drosophila melanogaster. To study the distribution of the predicted DAR-2 protein by immunocytochemistry, antisera were raised against a synthetic peptide corresponding to part of the amino terminus of the receptor sequence. In the brain of larval Drosophila, immunoreactivity appeared to be associated with glial septa surrounding neuropil compartments. In the ventral ganglion, immunoreactive cell bodies appeared to reside in the cortex of the ganglion, surrounding the central neuropil and neurohemal organs. In addition, double labeling immunocytochemistry revealed a substantial superposition between distribution of AST-like immunoreactivity and the putative DAR-2 protein in at least five cell bodies in the region of the ring gland corresponding to the corpora cardiaca.