Tandem mass spectrometry approach for the investigation of the steroidal metabolism: Structure-fragmentation relationship (SFR) in anabolic steroids and their metabolites by ESI-MS/MS analysis

► Correlating MS/MS behavior and the structural features of anabolic steroidal metabolites. ► Utilizing fungal culture as probable source of mammalian-like steroidal metabolites. ► Evaluating structural variation as potential difficulties in characterization. ► Previously non-available MS/MS data of some steroidal metabolites are also presented.