Combined quantification of corticotropin-releasing hormone, cortisol-to-cortisone ratio and progesterone by liquid chromatography-Tandem mass spectrometry in placental tissue

- LC-Tandem MS serves as a novel, multi-modal method of placental hormone analysis.
- CRH, cortisol/cortisone and progesterone can be quantified in the same sample.
- Villous sampling below the decidual basal plate showed the highest reliability.
- LC-Tandem MS can be used as a valid alternative for placental RIA and ELISA tissue assays.
- LC-Tandem MS might aid analyzing placental 11β-HSD2 enzyme activity.