کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10852674 1071982 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of an intravenous immunoglobulin with improved safety and functional activity
ترجمه فارسی عنوان
توسعه ایمونوگلوبولین داخل وریدی با فعالیت ایمنی و عملکردی بهبود یافته
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوشیمی، ژنتیک و زیست شناسی مولکولی (عمومی)
چکیده انگلیسی
The development and properties of a liquid intravenous immunoglobulin (Gammaplex®), of high purity, stability and functional activity, is described. Virus and TSE reduction by specific steps in the process were evaluated by spiking studies using small-scale models. The removal of procoagulant activity was determined using immunochemical and functional activity assays. Neutralisation and opsonic activity were used to demonstrate the functional activity of the IgG. The final low pH formulated product was stable at room temperature and was of high purity and functional activity. Three dedicated virus inactivation steps, i.e. solvent detergent, low pH and virus filtration, were shown to be effective. When combined with the B + I ethanol precipitation step, this gave a total reduction of >21 to >24 log for the enveloped and >10 to >13 log for the non-enveloped viruses tested. Several steps in the process were shown to contribute to TSE removal using scrapie. Potential procoagulant activity including Factor XI/XIa, was reduced to very low/undetectable levels in the final product. A new high purity liquid IVIG product has been developed, of high purity and good functional activity and stability. The process includes various steps for the removal of pathogens and procoagulant activity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biologicals - Volume 43, Issue 2, March 2015, Pages 123-129
نویسندگان
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