کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10869787 1073970 2015 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Structural insights into the loss of catalytic competence in pectate lyase activity at low pH
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Structural insights into the loss of catalytic competence in pectate lyase activity at low pH
چکیده انگلیسی
Pectate lyase, a family 1 polysaccharide lyase, catalyses cleavage of the α-1,4 linkage of the polysaccharide homogalacturonan via an anti β-elimination reaction. In the Michaelis complex two calcium ions bind between the C6 carboxylate of the d-galacturonate residue and enzyme aspartates at the active centre (+1 subsite), they withdraw electrons acidifying the C5 proton facilitating its abstraction by the catalytic arginine. Here we show that activity is lost at low pH because protonation of aspartates results in the loss of the two catalytic calcium-ions causing a profound failure to correctly organise the Michaelis complex.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 589, Issue 21, 24 October 2015, Pages 3242-3246
نویسندگان
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