کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10870055 1073988 2015 35 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Membrane protein production in Escherichia coli cell-free lysates
ترجمه فارسی عنوان
تولید پروتئین غشایی در لیزات سلولهای اشرشیاکلی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
چکیده انگلیسی
Cell-free protein production has become a core technology in the rapidly spreading field of synthetic biology. In particular the synthesis of membrane proteins, highly problematic proteins in conventional cellular production systems, is an ideal application for cell-free expression. A large variety of artificial as well as natural environments for the optimal co-translational folding and stabilization of membrane proteins can rationally be designed. The high success rate of cell-free membrane protein production allows to focus on individually selected targets and to modulate their functional and structural properties with appropriate supplements. The efficiency and robustness of lysates from Escherichia coli strains allow a wide diversity of applications and we summarize current strategies for the successful production of high quality membrane protein samples.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 589, Issue 15, 8 July 2015, Pages 1713-1722
نویسندگان
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