کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10870200 1073992 2014 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Phospholipase D2 downregulation induces cellular senescence through a reactive oxygen species-p53-p21Cip1/WAF1 pathway
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Phospholipase D2 downregulation induces cellular senescence through a reactive oxygen species-p53-p21Cip1/WAF1 pathway
چکیده انگلیسی
The expression of phospholipase D1 (PLD1) and PLD2 were found to decrease at the transcription level during both replicative and premature senescence in human lung fibroblast IMR-90 cells. Knockdown of PLD2 dramatically induced senescent phenotype in proliferating IMR-90 cells and wild-type HCT116 colon cancer cells, whereas this response was nearly abolished in p53- or p21Cip1/WAF1-null HCT116 cells. PLD2 knockdown increased the intracellular reactive oxygen species (ROS). Antioxidant N-acetyl-l-cysteine, NADPH oxidase inhibitor apocynin, and p22phox small interfering RNA (siRNA) reduced ROS generation and thus suppressed the appearance of senescence markers. Elevated CK2 α subunit (CK2α) expression repressed PLD2 downregulation-mediated senescence. PLD2 overexpression increased protein kinase CK2 (also known as casein kinase 2) (CK2) activity. Taken together, these results show that PLD2 downregulation causes senescence through the p53-p21Cip1/WAF1 pathway by stimulating ROS production, which is induced by CK2 inhibition.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 588, Issue 17, 25 August 2014, Pages 3251-3258
نویسندگان
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