کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10870202 | 1073992 | 2014 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Dynein-dependent transport of spindle assembly checkpoint proteins off kinetochores toward spindle poles
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
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![عکس صفحه اول مقاله: Dynein-dependent transport of spindle assembly checkpoint proteins off kinetochores toward spindle poles Dynein-dependent transport of spindle assembly checkpoint proteins off kinetochores toward spindle poles](/preview/png/10870202.png)
چکیده انگلیسی
A predominant mechanism of spindle assembly checkpoint (SAC) silencing is dynein-mediated transport of certain kinetochore proteins along microtubules. There are still conflicting data as to which SAC proteins are dynein cargoes. Using two ATP reduction assays, we found that the core SAC proteins Mad1, Mad2, Bub1, BubR1, and Bub3 redistributed from attached kinetochores to spindle poles, in a dynein-dependent manner. This redistribution still occurred in metaphase-arrested cells, at a time when the SAC should be satisfied and silenced. Unexpectedly, we found that a pool of Hec1 and Mis12 also relocalizes to spindle poles, suggesting KMN components as additional dynein cargoes. The potential significance of these results for SAC silencing is discussed.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 588, Issue 17, 25 August 2014, Pages 3265-3273
Journal: FEBS Letters - Volume 588, Issue 17, 25 August 2014, Pages 3265-3273
نویسندگان
PatrÃcia M.A. Silva, Rita M. Reis, Victor M. Bolanos-Garcia, Claudia Florindo, Álvaro A. Tavares, Hassan Bousbaa,