کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10870784 | 1074020 | 2013 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
miR-145 suppresses osteogenic differentiation by targeting Sp7
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موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: miR-145 suppresses osteogenic differentiation by targeting Sp7 miR-145 suppresses osteogenic differentiation by targeting Sp7](/preview/png/10870784.png)
چکیده انگلیسی
Osteogenesis depends on a coordinated network of transcription factors including Sp7. Emerging evidence indicates that microRNAs (miRNAs) act as pivotal regulators in various biological processes including osteoblast proliferation and differentiation. Here, we investigated the effect of miR-145 on osteogenic differentiation. miR-145 was decreased during osteogenic differentiation, which could suppress the osteogenic differentiation of C2C12 and MC3T3-E1 cells confirmed by gain- and loss-of-function experiments. Moreover, bioinformatic analysis combined with luciferase reporter assay, and Western blot validated that miR-145 negatively regulated Sp7 expression. Inhibition of Sp7 showed similar effect with miR-145 on osteogenic differentiation, whereas overexpression of Sp7 attenuated this effect. Collectively, these data indicate that miR-145 is a novel regulator of Sp7, and it suppresses the osteogenic differentiation of C2C12 and MC3T3-E1 cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 587, Issue 18, 17 September 2013, Pages 3027-3031
Journal: FEBS Letters - Volume 587, Issue 18, 17 September 2013, Pages 3027-3031
نویسندگان
Jie Jia, Qing Tian, Song Ling, Yong Liu, Shuhua Yang, Zengwu Shao,