The fission yeast Pvg1p has galactose-specific pyruvyltransferase activity

► N-Glycan from fission yeast contains pyruvic acid 4,6-ketal-linked galactose. ► Putative Schizosaccharomyces pombe pyruvyltransferase Pvg1p produced in Escherichia coli is characterized. ► Pvg1p transfers pyruvyl residues specifically to β-linked galactose.